Journal: Frontiers in Immunology

Article Title: Cytokines Induce Faster Membrane Diffusion of MHC Class I and the Ly49A Receptor in a Subpopulation of Natural Killer Cells

doi: 10.3389/fimmu.2016.00016

Figure Lengend Snippet: FCS measurement and representative autocorrelation curves . The autocorrelation curves of H-2D d and Ly49A were measured in freshly isolated murine NK cells after 4 h of cytokine stimulation. (A) A schematic illustration of the positioning of the focus (not to scale). FCS measurements were performed on the upper cell membrane. (B,C) Representative FCS autocorrelation curves for a control, an IL-2, and an IFN-α + β stimulated cell for (B) H-2D d entities, and (C) Ly49A entities. The cells were selected based on a location close to the center in the multivariate PCA analysis for each group (see for description of the PCA analysis).

Article Snippet: Natural killer cells were enriched from freshly isolated murine splenocytes by negative selection of all other cells, using MACS TM NK cell isolation kit mouse II (Miltenyi Biotec Norden AB , Sweden) according to the manufacturer’s recommendations.

Techniques: Isolation, Membrane, Control

Journal: Frontiers in Immunology

Article Title: Cytokines Induce Faster Membrane Diffusion of MHC Class I and the Ly49A Receptor in a Subpopulation of Natural Killer Cells

doi: 10.3389/fimmu.2016.00016

Figure Lengend Snippet: Diffusion rates of H-2D d and Ly49A are increased after 4 h of cytokine stimulation . The diffusion rates were measured in freshly isolated murine NK cells after 4 h of cytokine stimulation. (A) Diffusion rates of H-2D d in individual cells. (B) Diffusion rates of Ly49A. Cells in A and B are the same as in Table . The lines show Geometric mean, error bars show 95% confidence interval. (C–E) Linear regression of Ly49A and H-2D d diffusion coefficients in the same cells, 35, 32, and 33 cells for control, IL-2, and IFN-α + β, respectively. (C) Diffusion rate in control cells. (D) Diffusion rate in IL-2 stimulated cells. (E) Diffusion rate in IFN-α + β stimulated cells. * = 0.05 < p < 0.01, ** = 0.01 < p < 0.001, *** = p < 0.001 in A, B compared to control, in C–E for slope difference compared to 0. R 2 , least squares goodness of fit.

Article Snippet: Natural killer cells were enriched from freshly isolated murine splenocytes by negative selection of all other cells, using MACS TM NK cell isolation kit mouse II (Miltenyi Biotec Norden AB , Sweden) according to the manufacturer’s recommendations.

Techniques: Diffusion-based Assay, Isolation, Control